• Categoría: Artículo
  • Título: Establishment of callus and cell suspensions of wild and domesticated Opuntia species: study on their potential as a source of metabolite production
  • Autor (es): Marissa Robles-MartínezAna Paulina Barba-de la RosaFrancoise GuéraudAnne Negre-SalvayreMichel RossignolMaría del Socorro Santos-Díaz
  • Revista: Plant Cell, Tissue and Organ Culture (PCTOC)
  • Volumen: 124
  • Páginas: 181-189
  • Formato: Enlace
  • Subido por: Marissa

Establishment of callus and cell suspensions of wild and domesticated Opuntia species: study on their potential as a source of metabolite production

Marissa Robles-MartínezAna Paulina Barba-de la RosaFrancoise GuéraudAnne Negre-SalvayreMichel RossignolMaría del Socorro Santos-Díaz

Opuntia streptacantha and Opuntia megacantha are considered wild species, while Opuntia ficus-indica is deemed a domesticated species. Opuntia species have relevant nutritional and medicinal properties, related to their high levels of phenolics compounds.The content of phenolic acids and flavonoids, and antioxidant activity were all similar in callus and suspensions; however, growth rate was higher in cell suspensions in all species. Production of phenolic acids was correlated to the antioxidant activity, with these parameters being higher in O. streptacantha, the wild species. The concentration of metabolites in callus and cellular suspensions was about three times higher compared with cladodes, indicating that the use of in vitro cultures of Opuntia species is a viable alternative for the production of antioxidants under controlled conditions.