• Categoría: Artículo
  • Título: Exposure to silver nanoparticles produces oxidative stress and affects macromolecular and metabolic biomarkers in the goodeid fish Chapalichthys pardalis
  • Autor (es): Roberto Carlos Valerio-García, Ana Laura Carbajal-Hernández, Erika Berenice Martínez-Ruíz, Víctor Hugo Jarquín-Díaz, Catalina Haro-Pérez, and Fernando Martínez-Jerónimo
  • Revista: Science of the Total Environment
  • Volumen: 583
  • Páginas: 308–318
  • Formato: Enlace
  • Subido por: Catalina Ester

Exposure to silver nanoparticles produces oxidative stress and affects macromolecular and metabolic biomarkers in the goodeid fish Chapalichthys pardalis

Roberto Carlos Valerio-García, Ana Laura Carbajal-Hernández, Erika Berenice Martínez-Ruíz, Víctor Hugo Jarquín-Díaz, Catalina Haro-Pérez, and Fernando Martínez-Jerónimo

Silver nanoparticles (AgNPs) are the most commercialized nanomaterial worldwide, mainly due to their microbicidal
activity. Although, AgNPs have been shown to be toxic to aquatic species, their effect on endemic fish, like
Goodeidae, has not been demonstrated. Endemic species are under strong pressures by anthropogenic contamination
and destruction of their habitat; therefore, we studied adult Chapalichthys pardalis, an endemic fish of
Mexico. We evaluated the toxic effect of AgNPs through oxidative stress, macromolecular and metabolic biomarkers.
We determined the LC50 (96 h) and performed subchronic tests (21 days) using sublethal AgNPs concentrations
(equivalent to CL1 and CL10). At the end of the bioassay, we quantified 10 stress biomarkers in the
liver, gills, andmuscle, including the antioxidant enzymes (superoxide dismutase [SOD], catalase [CAT], and glutathione
[GPx]), thiobarbituric acid reactive species (TBARS), protein oxidation (C_O), macromolecules (proteins,
lipids, and carbohydrates), and metabolites (glucose and lactate). In addition, we determined the
integrated biomarkers response (IBR). LC50 was of 10.32 mg L−1. Results of subchronic exposure (21 days) revealed
that AgNPs produce oxidative stress in C. pardalis adults, as evidenced by a diminution in antioxidant enzymes
activity and an increase in TBARS and oxidized proteins. AgNPs also diminished levels of macromolecules
and generated a high-energy consumption, reflected in the reduction of glucose levels, although lactate levels
were not altered.